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Equilibrium buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris-HCl (pH 7.8) | 3.5 mM | 21.20 mg |
| NaCl | 2.5 mM | 7.31 mg |
| MgCl2 | 0.5 mM | 2.38 mg |
| ddH2O | N/A | 50 mL |
| Total | N/A | 50 mL |
This buffer needs to prepare fresh the day before or on the day of experiment and precooled at 4°C.
