REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
0.01 M PBS (powder, pH 7.2–7.4)	Solarbio Life Science	Cat#P1010
Phenylmethylsulfonyl fluoride (PMSF)	Sigma-Aldrich	Cat#P7626
Dehydrated alcohol (CH3CH2OH)	ANTE	N/A
Hydrochloric acid (HCl)	http://www.zh-chem.com	N/A
Trizma base	Sigma-Aldrich	Cat#V900483
Sodium chloride (NaCl)	Sigma-Aldrich	Cat#V900058
Magnesium chloride (MgCl2)	Sigma-Aldrich	Cat#208337
Trypan blue stain (0.4%)	Gibco	Cat#15250061
Sucrose	Sigma-Aldrich	Cat#V900116
Ethylenedinitrilotetraacetic acid (EDTA)	Sigma-Aldrich	Cat#V900106
Potassium chloride (KCl)	Sigma-Aldrich	Cat#P5405
Sodium phosphate dibasic (Na2HPO4)	Sigma-Aldrich	Cat#V900061
Sodium carbonate (Na2CO3)	Sigma-Aldrich	Cat#S7795
Proteinase K	Beyotime Biotechnology	Cat#ST533
Triton X-100	Sigma-Aldrich	Cat#93443
Experimental models: Cell lines
Cultured cells, e.g., HEK293T cells	Stem Cell Bank, Chinese Academy of Sciences	Cat#BFN60700191
Software and algorithms
BioRender	BioRender.com	N/A
Other
Cell culture dish	Sigma-Aldrich	Cat#CLS430167
Cell lifer	Sigma-Aldrich	Cat#CLS3008
15 mL centrifuge tube	Sigma-Aldrich	Cat#430791
Eppendorf 5424R Microcentrifuge	Eppendorf	Cat#5406000119
Vent filter for reservoir	Pall Life Sciences	Cat#LWFS32501
15 mL Dounce tissue grinder	Duran Wheaton Kimble Life Science	Cat#357544
Nikon Eclipse 80i fluorescence microscope	Nikon	N/A
1.5 mL centrifuge tube	Maisinuo	Cat#HZX018-2
Scientz-IID ultrasonic homogenizer	Ningbo Scientz Biotechnology	N/A
Type 42.2 Ti Rotor	Beckman Coulter	Cat#343007
Open-top thickwall polypropylene tube	Beckman Coulter	Cat#343621
Beckman Coulter Optima XPN-100 ultracentrifuge	Beckman Coulter	Cat#A94469
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Cat#23225
SpectraMax iD3 Multi-Mode Microplate Reader	Molecular Devices	N/A
PowerPac Universal power supply	Bio-Rad	Cat#1645070
Protec OptiMax 2010-NDT tabletop film processor	Protec	N/A
X-OMAT BT Film (5 × 7 in.)	Carestream	N/A
Epson Perfection V850 Pro photo scanner	Epson	Cat#B11B224201