Table 2.
Primers used for quantitative RT-PCR.
| Primer name1 | Primer sequence2 (5′-3′) | Product size (bp) | GenBank accession number |
|---|---|---|---|
| GAPDH | F: tgctgcccagaacatcatcc | 142 | NM_204305 |
| R: acggcaggtcaggtcaacaa | |||
| CPT1A | F: tctcatgctttggaaagaa | 147 | NM_001012898 |
| R: attcgctgttcagaagagtt | |||
| PPAR-α | F: gtaagctctcagaaactttgtt | 145 | NM_001001464 |
| R: ccgctttccataatctgttc | |||
| FASN | F:ttcccaaaaagggatcaaca | 132 | NM_205155.3 |
| R: agttcttcagagcatcgttt | |||
| SREBP-1 | F: gccctctgtgcctttgtcttc | 130 | AY_029224 |
| R: actcagccatgatgcttcttcc | |||
| ACC | F: aatggcagctttggaggtgt | 117 | NM_205505 |
| R: tctgtttgggtgggaggtg | |||
| SCD | F: gatactacaagccctcagtg | 132 | NM_204890 |
| R: cattgagccctaaggtgtag | |||
| PPAR-γ | F: ccagcgacatcgaccagtt | 145 | AF_163811 |
| R: ggtgatttgtctgtcgtctttcc | |||
| Malic enzyme | F: tgccagcattacggtttagc | 175 | NM_204303 |
| R: ccattccataacagccaaggtc |
1
GAPDH, glyceraldehyde-3-phosphate; CPT1A, carnitine palmitoyl transferase 1A; PPAR-α, peroxisome proliferator-activated receptor-α; FASN, fatty acid synthase; SPEBP-1, sterol response element-binding protein 1; ACC, acetyl-CoA carboxylase; SCD, stearoyl-CoA desaturase; PPAR-γ, peroxisome proliferator-activated receptor-γ.
2
F, forward; R, reverse.