Figure 4.

Constitutive expression of Pdx1 partially rescues pancreas weight and β-cell mass. Representative images of pancreas, stomach, spleen, and duodenum at birth showing varying degrees of pancreatic hypoplasia in OgtKO^Panc and OgtKO^Panc;Pdx1Tg^Panc (A, scale bar represents 250 pixels). Body weight (B, n = 6–19), pancreas weight (C, n = 6–73), random blood glucose (D, n = 6–18), β-cell mass (E, n = 5–9), α-cell mass (F, n = 5–9), and exocrine cell mass (G, n = 5–9) at birth. Representative images of whole pancreas (H, 10× magnification, scale bar represents 500 μm; areas outside dotted lines indicate space added from canvas size adjustment) and islets (I, 40× magnification, scale bar represents 50 μm; areas outside dotted lines indicate space added from canvas size adjustment). Statistics conducted using one-way ANOVA followed by unpaired Student t test with Welch’s correction, p ≤ 0.05 deemed significant. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.