Fig. 7.

Intranasal administration of GS³⁴SSG increased levels of³⁴S-labeled GSSSG and ratios of³⁴S-labeled sulfane sulfur species to endogenous sulfane sulfur species in CNS. A. Amount of ³⁴S-labeled GSSSG detected in OB + FB, BS, C + ThSC, and LSC at 30 min after intranasal administration of 50 mg/kg of GS³⁴SSG. n = 4 mice for OB + FB, BS, and C + ThSC. n = 3 mice for LSC. Data are presented as means with standard deviation. B. Ratios of [³²S, ³⁴S]GSSH to [³²S₂]GSSH, [³²S, ³⁴S]CysSSH to [³²S₂]CysSSH, and [³²S₂, ³⁴S]CysSSSCys to [³²S₃]CysSSSCys in OB + FB, BS, C + ThSC, and LSC at 30 min after intranasal administration of 50 mg/kg of GS³⁴SSG. n = 4 mice for each organ. Data are presented as means with standard deviation for the ratios of [³²S, ³⁴S]GSSH to [³²S₂]GSSH and [³²S₂, ³⁴S]CysSSSCys to [³²S₃]CysSSSCys, and as medians with interquartile range for the ratio of [³²S, ³⁴S]CysSSH to [³²S₂]CysSSH.

Abbreviations: BS, brainstem; CNS, central nervous system; C + ThSC, cervical and thoracic spinal cord; CysSSH, cysteine hydropersulfide; CysSSSCys, cysteine trisulfide; GSSH, glutathione hydropersulfide; GSSSG, glutathione trisulfide; LC-MS/MS, Liquid chromatography-tandem mass spectrometry; LSC, lumbar spinal cord; OB + FB, olfactory bulb and forebrain.