Fig. 8.

GSSSG increased relative sulfane sulfur levels in lumbar spinal cord and primary cortical neurons. A. Relative sulfane sulfur levels in lumbar spinal cords at 48 h after surgery in mice subjected to a sham surgical procedure, or SCI and treated with GSSSG or vehicle alone. Relative sulfane sulfur levels in lumbar spinal cords were estimated using a fluorescent probe, SSip-1. n = 6 mice for each group. Data are presented as means with standard deviation. B. Relative sulfane sulfur levels in primary cortical neurons after incubation with GSSSG at 10 μM, Na₂S₃ at 10 μM, or vehicle alone. Data were analyzed using one-way ANOVA with Dunnett's multiple comparison test. n = 10 for GSSSG and vehicle groups. n = 5 for Na₂S₃ group. Data are presented as means with standard deviation.

Abbreviations: GSSSG, glutathione trisulfide; Na₂S₃, sodium trisulfide; SCI, spinal cord ischemia.