Fig. 1.

Serum opacity factor reaction. A: Kinetics of reaction versus HDL from WT and Scarb1^−/− mice as labeled. Dashed and solid lines are the experimental data and the data fitted lines, respectively, to a rising exponential fit. HDL (1 mg/ml) and SOF (1 μg/ml) were mixed at 37°C, and opacification was monitored by right-angle light scattering. B–E: SEC analysis of aliquots of the SOF reaction products versus HDL that were collected after completion of the kinetic studies. SOF incubated with HDL from WT (B, D) and Scarb1^−/− (C, E) mice. Profiles are expressed as protein absorbance (280 nm, B, C) and cholesterol concentration (D, E). The profiles with red fill are the protein absorbance (280 nm) profiles of HDL before treatment with SOF. SOF, serum opacity factor; SEC, size-exclusion chromatography.