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. 2023 Feb 2;14:1057394. doi: 10.3389/fphys.2023.1057394

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Copyright © 2023 Hanly, Johnston, Lemass, Jose, Tornifoglio and Lally.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Establishment of the optimum concentration for PTA penetration through arterial tissue samples. (A) Native control (B) Stained sample for 22 h at 1% PTA (C) Preservation in ethanol for 2 weeks (D) Removal of stain using NaOH for 22 h (E) Stained sample for 12 h at 0.3% PTA (F) Removal of stain using NaOH for 12 h. (G) Stained sample for 18hrs in 0.05% stain. (H) Removal of stain using NaOH for 18 h.