. 2023 Feb 2;14:1030414. doi: 10.3389/fmicb.2023.1030414

Table 2.

List of primers and accession number of Arabidopsis sequences used in real-time RT-PCRs in the present study.

Gene	Forward primer 5’–3’	Reverse primer 5’–3’	nM	NCBI accession no.
UBC9 ^a	TCACAATTTCCAAGGTGCTGC^b	CGAGCAGTGGACTCGTACTT^b	300	NM_179131.3
UBC9 ^a	TCACAATTTCCAAGGTGCTGC^b	CGAGCAGTGGACTCGTACTT^b	300	NM_118934.3
TIP41	CCTCTTGCGATTTTGGCTGAG^b	ACGAAGAACAGTTGGTGCCT^b	300	NM_119592.5
SAND	AGATCAATCGCGGAAGGTGG^b	TATGTCGGGACCAGGTGAGT^b	300	NM_128399.4
UBQ10 ^a	CGTCTTCGTGGTGGTTTCTAA^b	ACAAGGCCCCAAAACACAAAC^b	300	NM_178968.5
				NM_001084884.5
				NM_001340546.1
				NM_116771.5
				NM_202787.4
				NM_001340547.1
				NM_178969.6
				NM_178970.5
MXI-K ^a	ACAGCCATTGAAGTGCCAGA	TTCGCCTCTGCGGTGTTAAA	300	NM_001161252.2
				NM_001343670.1
				NM_001343671.1
MXI-1	AGACGTGAATGCTGCTCGTT	AGCCGAACCAACAAACTCCT	300	NM_101620.3
MXI-2 ^a	ACTCCAAGCAGCCAAGAACA	TCCAGGTCAGTCCTTATCCGT	300	NM_001203536.1
				NM_123757.5
				NM_001203536.1
bZIP17	GCTCTATCCTCTGGCTCTGC	ATGGGACCTGCAACACCTTC	300	NM_129659.3
bZIP28	TTCCCGGATCTTTGTGGTGG	TCAGGTGGCTACGAGATGGA	300	NM_111917.5
bZIP60u	AGGAGTCTGCTGTGCTCTTG	TCTGGACGTAGGAGGCAACA	300	NM_103458.3
bZIP60s	GAGTCTGCTGTTGGGTTCCC	TCTGGACGTAGGAGGCAACA	300	NM_103458.3
IRE1A	AAAGTTTTCGTCGAGGGGCA	TCCTTCGCGGATTTACGGTT	300	NM_127306.4
IRE1B ^a	ATTTGAGACCGAGAGCACAAG	TATCGCTTGCATCCCGAAGA	300	NM_001203453.2
				NM_122344.5
				NM_001203454.1
S1P ^a	AGGCATCAAAGGAAGACCCTG	CAGGAGCCAGTAGCAGTTGG	300	NM_121971.3
				NM_001343619.1
				NM_001343620.1
S2P ^a	TGTGGTGATGGATGGGTGAC	AGTCACCCTGTGGACATACG	300	NM_001341415.1
				NM_001341416.1
				NM_001341418.1
				NM_001341412.1
				NM_001341413.1
				NM_001160784.2
				NM_001341417.1
				NM_001341414.1
BIP1/2 ^a	AGGACTTTGACCACAGGATCA	TGCCCTCTCACATTCCCTTC	300	NM_122737.4
				NM_001344430.1
				NM_180788.3
				NM_123567.3
BIP3 ^a	CAAGGAACCCAGCAAAGGGA	GGCGCAACATCAAGCAGTAG	300	NM_001198015.2
BIP3 ^a	CAAGGAACCCAGCAAAGGGA	GGCGCAACATCAAGCAGTAG	300	NM_100779.5
PDI1 ^a	CCACTACCGGAAAATAACGATGG	GGCCACACCAAGGAGCATA	300	NM_001125370.1
PDI1 ^a	CCACTACCGGAAAATAACGATGG	GGCCACACCAAGGAGCATA	300	NM_115353.5
PDI5 ^a	CATCCAACAAGGGACAGGGT	GTCCAAAGTACTGGAATGCACC	300	NM_102024.4
PDI5 ^a	CATCCAACAAGGGACAGGGT	GTCCAAAGTACTGGAATGCACC	300	NM_179365.1
CNX1	GGTCTCAAGAGCTACCAGAAGG	GTTTGGTTGTTGCTCGGCTT	300	NM_125573.4
CNX2 ^a	CCACACGAATCTTTTCAGTCCA	TCGCAAGTGAATTTGTTGTTGTT	300	NM_001336358.1
				NM_001036384.2
				NM_179846.4
				NM_001336359.1
				NM_001336357.1
CRT1 ^a	AAGCACAAGGATGCGGAGAA	CTTCCTCAGCGTCGGATTCA	300	NM_104513.5
CRT1 ^a	AAGCACAAGGATGCGGAGAA	CTTCCTCAGCGTCGGATTCA	300	NM_001036122.2
CRT2	AAGCTCAAGGATGCGGAGAA	CAGCATCAGATTCCGCAGGT	300	NM_100791.4
CRT3 ^a	CCGGTATGGAGACAGGAGGA	GCCTCATAGCTCGTCATGGT	300	NM_100718.5
				NM_001198007.1
				NM_202064.1
SYT1	CGGTCAGAGATCCCCAGACT	TCTCGGGATTCCCAACCTGT	500	NM_127668.4
VAP27-1	AGAGACGGGGTGGAGAGAAT	AACTGCAACGTTCGTGGTTG	500	NM_115924.4
NET3C	GCATTGAGGTCTCCTTTGCG	TCCCTAACACAACAACATATCCCA	500	NM_001337284.1

This primer pair amplifies every gene transcript variant.

Pagliari et al. (2017).