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. 2023 Feb 2;14:1113932. doi: 10.3389/fimmu.2023.1113932

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Copyright © 2023 Lesteberg, Araya, Waugh, Chauhan, Espinosa and Beckham

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Severe COVID-19 patients have more CD4+ CD62L+ Tregs. The frequencies of CD4+ and CD8+ Tregs (viable, CD14- CD56-, CD19-, CD3+, CD4+ or CD8+, CD127lo, CD25+ FoxP3+) in non-severe and severe COVID-19 patients, recovered COVID-19 patients, and healthy controls were assessed by flow cytometry. (A) Example flow cytometry staining showing CD4+ CD62L+ T cells being gated on CD127lo, followed by gating for CD25+ FoxP3+ to identify Tregs. (B) CD4+ CD62L+ Tregs; (C) CD8+ CD62L+ Tregs. *p<0.05 one way ANOVA with multiple comparisons correction.