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. 2023 Feb 2;14:1067505. doi: 10.3389/fmicb.2023.1067505

Figure 4.

Figure 4

Effect of pasteurized E. coli Nissle on EcNC101 growth, tumorigenesis and tumor development in inulin-fed ApcMin/+ mice. (A) Competition assay between EcNC101 and EcN for three days (Fisher’s Test). (B) OD600 of EcNC101 grown in LB supplemented with 50 and 100% pasteurized EcN (repeated-measure ANOVA). (C–I) Mice were fed a diet supplemented with 10% inulin and were gavaged with PBS or 108 CFU of EcNC101 at W2, followed by an oral gavage with PBS or 108 CFUs of pasteurized EcN (PEcN) at W3, 4 and 5. (C–E) EcNC101 colonization assessed by (C) PCR quantification of clbP gene level normalized to 16S rRNA gene levels; and by CFUs in the (D) small intestine and (E) in the colon. (F) Body weight (mean ± SEM; between PBS and EcNC101 mice, repeated-measure ANOVA). (G) Tumor count in the small intestine. (H) Representative images of colonic tumors (left side: cecum, right side: rectum), scale bar = 1 cm. (I) Tumor size in the colon. (J) Colonic tumor grades (Chi-square Test). n.s. non-significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ANOVA, N = 9–14 per group.