Reproducibility is dependent upon all investigated effects, and each bundle segmentation methods is affected differently. Effects of scan-rescan (RESCAN; blue), scanners (SCAN1, SCAN2; red), vendor (VEN1, VEN2; dark purple), resolution (RES1, RES2; pink), diffusion directions (DIR1, DIR2; green) and b-value (BVAL; light purple) on dice overlap coefficient for all fiber bundles dissected using each technique. For each, a Wilcoxon signed rank test is performed to investigate differences in effects. Statistically significant results (p <.05/45/4 comparisons) are shown as a solid line, and those not reaching statistical significance are shown as dashed line. Tractseg (top-left), ATK (top-right), Reco (bottom-left), and Xtract (bottom-right).