Table 1. HILIC Method Validation Results for Imprecision, LOQ, Linearity, and Carryover.

analyte	average RSD (% CV)	LOQ (μM)	linearity (R2)	carryover (%)
CRN	8.9	11.2	0.996	0.0014
Val	15	115	0.993	0.059
t-Hcy	20	6.85	0.985	0.025
GUAC	6.1	0.456	0.996	–0.056
Leu	5.5	24.2	0.997	0.061
CRE	6.1	115	0.989	0.014
MA	7.2	4.29	0.997	0.16
MMA	8.6	1.51	0.997	0.47
EMA	6.1	0.867	0.997	0.032
3-HGA	10	0.753	0.998	0.50
2-HGA	5.4	9.65	0.993	0.12
OA	5.6	0.297	0.998	0.026
MCA	5.7	0.305	0.998	0.029
LPC 20:0	8.2	0.0809	0.998	0.032
LPC 22:0	7.5	0.0159	0.998	0.00018
LPC 24:0	9.3	0.0294	0.991	0.0051
LPC 26:0	14	0.0264	0.992	0.010
Ile	11	9.85	0.994	0.050
aIle	11	0.347	0.992	0.065