Figure 7.

Bacterial composition of the faeces of mice colonised with the faecal microbiota of patients before and after bioliopancreatic diversion with duodenal switch (BPD-DS) or laparoscopic sleeve gastrectomy (LSG). (A, B) Principal coordinate analysis (PCoA) on Bray-Curtis dissimilarity index comparing the faecal bacterial composition of female germ-free (GF) mice colonised with the faecal microbiota of patients before and after BPD-DS or LSG. The samples are stratified by patient donors of faecal microbiota. Permutational multivariate analysis of variance was used to assign statistical significance to the distance between clusters (presurgery vs postsurgery) in PCoA scatter plots. (C) Heat maps depicting the average of replicates for taxa significantly different between presurgery and postsurgery groups (p<0.05, Wilcoxon rank-sum test). ASVs were clustered at 99% similarity and collapsed to the genus level. The relative abundance of taxa is expressed as log₁₀ fold change from its median level across the entire cohort (both surgeries combined). All relative abundance values of 0 were assigned 1×10⁻⁶, an order of magnitude lower than the lowest detectable relative abundance in the data, to allow the logarithmic transformation of the fold change. Each square, triangle and circle represents a biological replicate (n=11–12).