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. 2023 Feb 16;11(2):e782. doi: 10.1002/iid3.782

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© 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Treatment of GA‐regulated Th17 and Treg cell differentiation balance in the mouse model of arthritis induced by collagen. Splenocytes were isolated (CD4+ T cell) and the frequencies of Th17 (CD4+IL‐17+) and Treg (CD25+FOXP3+, gated on CD4+ cells) were analyzed by using flow cytometry. The graphs represented the percentage of IL‐17A+CD4+ T cells (A) and FOXP3+CD25+ T cells (B). The data were shown as the means ± SD (n = 6). *p < .05, **p < .01, and ***p < .001 as compared to the control. ^# p < .05 and ^## p < .01 as compared to the CIA group. GAH corresponded to the GA administration dosage of 80 mg/kg/d.