Figure 5.

Inhibition of LPS-induced NO and ROS generation by UaB in zebrafish larvae. Zebrafish at 3 dpf were microinjected with 2 nL of 0.5 mg/mL LPS and placed in E3 media containing the indicated concentrations of UaB for 24 h. The larvae were incubated with 5 μM DAF-FM-DA (A and B) or 20 μM DCF-DA (C and D) for NO and ROS detection, respectively, and visualized using the CELENAS Digital Imaging System. Relative fluorescence intensities were calculated and expressed compared to the untreated control (B and D). Each value indicates the mean ± SD and is representative of three independent experiments with 10 fish for each group. Significant differences among the groups were determined (***p < 0.001, vs LPS-unstimulated larvae; ^##p < 0.01 and ^###p < 0.001, vs LPS-stimulated larvae).