Table 1.
Functional validation of three candidate desiccation-responsive genes. Expression level and survival of gene disruption and RNAi reciprocal crosses relative to the control strains (F = female and M = male). At least three replicates were analyzed in all cases (Additional file 8: Table S7C)
| Gene name/Flybase ID | Gene function | Gene disruption/RNAi (stock number) | qRT-PCR results (one-tailed t-test p-value) | Survival of the gene disruption/RNAi strain compared with background strain | Log-rank test (p-value) |
|---|---|---|---|---|---|
| nclb/ FBgn0263510 | Regulation of gene expression | P-element insertion in first intron (#21,138) | Lower expression (0.00190) | Higher survival | < 0.0001 |
| RNAi (#41826) F x Gal4-6c M | Lower expression (0.0139) | Higher survival | < 0.0001 | ||
| RNAi (#41826) M x Gal4-6c F | Lower expression (0.03.39) | Higher survival | < 0.0001 | ||
| Nsun2/ FBgn0026079 | RNA methylation, tRNA methylation | P-element insertion in first intron (#33452) | Lower expression (0.01.19) | Lower survival | < 0.0001 |
| RNAi (#62495) F x Gal4 M | Lower expression (0.0003) | Higher survival | < 0.0001 | ||
| RNAi (#62495) M x Gal4 F | Lower expression (0.0134) | Higher survival | < 0.0001 | ||
| Dbp73D/ FBgn0004556 | RNA binding | RNAi (#36131) F x Gal80ts M | Lower expression (0.00374) | Lower survival | < 0.0001 |
| RNAi (#36131) M x Gal80ts F | Lower expression (0.00461) | Lower survival | < 0.0001 |