Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 15;23:27. doi: 10.1186/s12935-023-02866-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Overexpression of CCL14-AS suppresses invasiveness and lymph node metastasis of CRC cells. A A basal level of CCL14-AS was determined using qRT-PCR assay in the non-malignant human colon epithelial cell line NCM460 and several CRC cell lines (including SW480, SW837, SW620, HCT116, DLD-1, LoVo, COLO205 and RKO). B qRT-PCR analysis was performed to detect the expression levels of CCL14-AS in LoVo and SW620 cells with or without CCL14-AS stable overexpression. C Wound healing assay showing cell migration of LoVo(left) and SW620(right) cells with or without CCL14-AS overexpression. Scale bar: 100 μm. D Migration and invasion of LoVo (left) and SW620 cells (right) with or without CCL14-AS overexpression were evaluated by using transwell assays. Scale bar: 500 μm. E Popliteal lymph nodes volume and size were significantly decreased in the CCL14-AS overexpression group compared with the control group. F Representative images of the lymph node stained with H&E(left) in the CCL14-AS overexpression group. Scale bar: 100 μm. Statistical results of the metastatic ratios of lymph nodes in the Vector control and CCL14-AS-Overexpresed group (right). * p < 0.05, ** p < 0.01, *** p < 0.001