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. 2023 Feb 15;54:12. doi: 10.1186/s13567-023-01144-2

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Overview of experimental design and RNA-seq data analysis pipeline. RNA was isolated from enteroids at 1, 3, 4 and 7 days and the freshly isolated ED18 intestinal villi (0 h). The validated RNA was sequenced on a Illumina NovaSeq 6000 system. All RNA-seq reads were preprocessed for a quality assessment. The filtered transcriptome reads were aligned to the genome and the expression profile was analysed.