Table 2.
Antibodies used in quantitative Western blot assays.
| Target and corresponding GAPDH reference | Primary antibody (supplier; dilution used) | Secondary antibody (antibodies purchased in LI‐COR Biosciences; used in 1:15,000 dilution) |
|---|---|---|
| P4HA1 |
Rabbit anti‐P4HA1; LSBio Inc.; 1:500 |
Anti‐rabbit (IRDye800CW) |
| GAPDH |
Mouse anti‐GAPDH; Santa Cruz Biotechnology Inc.; 1:1000 |
Anti‐mouse (IRDye680RD) |
| HSP47 |
Mouse anti‐HSP47; Santa Cruz Biotechnology Inc.; 1:500 |
Anti‐mouse (IRDye800CW) |
| GAPDH |
Chicken anti‐GAPDH; Abcam; 1:2000 |
Anti‐chicken (IRDye680RD) |
| CTGF |
Rabbit anti‐CTGF; Abcam; 1:1000 |
Anti‐rabbit (IRDye800CW) |
| GAPDH |
Mouse anti‐GAPDH; Santa Cruz Biotechnology Inc.; 1:1000 |
Anti‐mouse (IRDye680RD) |
| αSMA |
Mouse anti‐αSMA; Abcam; 1:500 |
Anti‐mouse (IRDye800CW) |
| GAPDH |
Chicken anti‐GAPDH; Abcam; 1:2000 |
Anti‐chicken (IRDye680RD) |
| Collagen I |
Rabbit anti‐Col1 (R69); in house; 1:2000 |
Anti‐rabbit (IRDye800CW) |
| GAPDH |
Mouse anti‐GAPDH; Santa Cruz Biotechnology Inc.; 1:1000 |
Anti‐mouse (IRDye680RD) |
| Fibronectin |
Mouse anti‐fibronectin; Santa Cruz Biotechnology Inc.; 1:500 |
Anti‐mouse (IRDye800CW) |
| GAPDH |
Chicken anti‐GAPDH; Abcam; 1:2000 |
Anti‐chicken (IRDye680RD) |
Abbreviations: CTGF, connective tissue growth factor; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; HSP47, heat shock protein 47; P4H, prolyl 4‐hydroxylase; αSMA, α smooth muscle actin.