Figure 4.

Effect of miR396a-5p concentration on the virulence inhibition. A) Tobacco leaves were inoculated with B cinerea conidiospores containing 5 μM, 10 μM and 20 μM ss-miR396a-5p. Partial necrosis were stained by trypan blue method. B) Tobacco leaves were inoculated with B cinerea conidiospores containing 5 μM, 10 μM and 20 μM ds-miR396a-5p. Partial necrosis were stained by trypan blue method. C) Diameters of the necrosis on tobacco leaves. Results are expressed as means ± SD of 3 biological replicates.