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. 2023 Feb 2;14:1072181. doi: 10.3389/fpls.2023.1072181

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Copyright © 2023 Wu, Huang, Jiang and Jin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of target gene of miR396a by transient co expression. (A) Overexpression vector constructs of miR396a-5p and its 2 target genes in B cinerea (Bcin15g02590.2 and Bcin04g04920.1). Gray small triangle: refers to the deletion of the target site. (B) Relative transcription levels of the 2 target genes co-expressed with miR396a-5p in N. benthamiana. Results are expressed as means ± SD of 3 biological replicates. Asterisks indicate a significant difference (*P < 0.05) compared to the corresponding the transient expression of the wild type target gene.