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. 2022 Mar 17;2022:gigabyte46. doi: 10.46471/gigabyte.46
Reviewer name and names of any other individual's who aided in reviewer Hai-Zhong Yu
Do you understand and agree to our policy of having open and named reviews, and having your review included with the published papers. (If no, please inform the editor that you cannot review this manuscript.) Yes
Is the language of sufficient quality? Yes
Please add additional comments on language quality to clarify if needed
Are all data available and do they match the descriptions in the paper? Yes
Additional Comments
Are the data and metadata consistent with relevant minimum information or reporting standards? See GigaDB checklists for examples <a href="http://gigadb.org/site/guide" target="_blank">http://gigadb.org/site/guide</a> Yes
Additional Comments
Is the data acquisition clear, complete and methodologically sound? No
Additional Comments
Is there sufficient detail in the methods and data-processing steps to allow reproduction? No
Additional Comments
Is there sufficient data validation and statistical analyses of data quality? Yes
Additional Comments
Is the validation suitable for this type of data? No
Additional Comments
Is there sufficient information for others to reuse this dataset or integrate it with other data? Yes
Additional Comments
Any Additional Overall Comments to the Author The manuscript presented by Shippy et al. revealed that chitinase family genes in Diaphorina citri. Chitin is widely distributed in nature and serves a variety of functions. In insects, chitin is a major structural component of the cuticle and peritrophic membrane, and plays an important role in molting; thus, chitin metabolism related genes can serve as a desired target for pest control. As described in background, chitinase plays an important role involved in digesting the polysaccharide polymer chitin. In the current study, the authors identified and annotated 12 chitinase family genes from D. citri and performed phylogenetic analysis. Additionally, the structural domains and expression patterns of D. citri chitinase genes were analyzed. In general, the manuscript can provide some useful information for D. citri control. This manuscript can be accepted after solving the following questions. 1. According to Table 1, 12 chitinases were identified, including CHT3, CHT5-7, CHT10-1, CHT10-2, CHT11, IDGF1-3, ENGase and CHTPE. However, CHT1-2, CHT4 and CHT8-9 seem to be missing. Please give a proper explain. 2. I suggested that the author should verify the expression levels of these chitinase genes by qPCR or Western blot.
Recommendation Major Revision