FIG 5.

Pgp3 and Pgp4 are required for plasmid-associated infectivity. Competition assays with starting ratios of 1:10 (left) or 1:30 (right) for CTE273:CTE3024 GFP (A), CTE277:CTE3024 mCherry (B), or CTE277:CTE273 (C) showed strains expressing Pgp3 and Pgp4 outcompeted strains expressing Pgp3 or Pgp4 alone. GFP-expressing and mCherry-expressing inclusions were quantified following each passage, and ratios were calculated. Data points represent mean ratios ± SD from two replicates. Competitive indices are means ± SD. Graphs are representative of two experiments. Slopes of the lines were compared to 0: P < 0.0001 (1:10 uninduced, 1:30 uninduced), P = 0.007 (1:10 induced), P = 0.027 (1:30 induced) (A); P < 0.0001 (1:10 uninduced, 1:10 induced, 1:30 uninduced, and 1:30 induced) (B); P = 0.31 (1:10 uninduced), P < 0.0001 (1:10 induced, 1:30 induced) P = 0.6873 (1:30 uninduced) (C); or to each other: ****, P < 0.0001 (1:10, 1:30) (A); **, P = 0.0007 (1:10), ns, P = 0.22 (1:30) (B); ****, P < 0.0001 (1:10, 1:30) (C); using simple linear regression. ns, not significant; UI, uninduced; Ind., induced.