FIG 1.

S. sputigena adherence to TIGKs is more abundant than to HIGKs. S. sputigena was fluorescently labeled with CellBright-488 prior to incubation with HIGKs or TIGKs on glass coverslips at MOIs of 10, 50, 100, and 200 for 4 h. Attachment of S. sputigena was measured by flow cytometry. (A) Representative histograms are shown for S. sputigena binding to HIGKs and TIGKs. (B) The results are the averages of 3 biological replicates, each with >50,000 HIGKs or TIGKs, with standard deviations. Treated cells were compared to untreated controls by one-way analysis of variance (ANOVA) with Dunnett’s post hoc test (**, P < 0.01; ****, P < 0.0001; ns, not significant).