Figure 1.

IL-27 effect on the generation/maintenance of IL-17-producing T cells. (A) Ex vivo sorted resting Teff cells (CD4⁺ CD25^- CD62L^- or (B) ex vivo sorted Treg (CD4⁺ CD25^high) from B27-rats aged of 3-5 months were cocultured with CD103⁺ DCs for 3 days; (C, D) ex vivo sorted naïve (CD4⁺ CD25^- CD62L⁺) T cells were cocultured with CD103⁺ DCs for 6 days in (C) Treg (TGF-β) or (D) Th₁₇ (TGF-β + IL-6 + IL-1β + IL-23) polarizing conditions. Left panels show representative flow cytometry dot plots evaluating the percentage of IL-17-producing CD4⁺ T cells after coculture and the mean + SEM of 6-11 rats/group; right panels show the mean + SEM of IL-17 and IL-10 levels that were evaluated in the supernatant of cocultures by ELISA. *p < 0.05; **p < 0.01 ; ***p = 0.0001..