FIG 1.

Multiplexed assay for schistosome viability. (A) Drug screening workflow on adult S. mansoni. Worms are harvested from mice, exposed to test compounds for 24 h (3 columns or 32 worms/drug), supplemented with 5-HT and imaged to record movement (15 sec/well). Media was changed to fresh drug-free media for follow-up assay measuring lactate production. (B) Quantification of movement for worms exposed to either DMSO control, dichlorvos (DDVP, 10 μM), or meclonazepam (MCLZ, 10 μM). Worms were imaged before (open symbols) or after (closed symbols) serotonin addition (5-HT, 250 μM). (C) Flow cloud showing worm movement over the period of recording for the plate. Yellow areas indicate worm movement, while dark wells remain paralyzed. Below = brightfield images of an individual worm representative of each treatment. (D) Top = plate media after culture (24 h in 5-HT, 250 μM) to allow accumulation of lactate. Bottom = measurement of lactate from the same plate using the Lactate-Glo assay system.