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. 2023 Jan 26;67(2):e01331-22. doi: 10.1128/aac.01331-22

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Copyright © 2023 Sow et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — NPP3C and 6A1HI inhibit the viral RNA replication step of DENV life cycle. (A) Huh7.5 were infected with reporter virus DENV-R2A and treated with DMSO (negative control), NITD008 (10 μM, positive control), NPP3C (50 μM), and 6A1HI (50 μM), added at different time points. At 2 days postinfection, Rluc assays were performed to measure viral replication. The results are representative of two independent experiments, each including biological duplicates. (B) Schematic representation of the modified DENV genomes which were electroporated in C–E. (C–E) Huh7.5 were electroporated with in vitro-transcribed (C) DENV-R2A full RNA genome, (D) sg-DENV-R2A subgenomic replicon, or (E) sg-DENV-R2A GND replication-incompetent subgenome and immediately treated with DMSO, 50 μM NPP3C, 50 μM 6A1HI, or 10 μM NITD008. At the indicated time points, transfected cells were subjected to Rluc assays. Results shown in C–E are representative of three independent experiments.