FIG 3.

Complementation of pbpA from JH2-2 (LS311), LS4828 (LS310), or the pBSU100 vector alone in the ΔpbpA strain is confirmed by Western blotting of E. faecalis strains grown in BHI broth to exponential phase. Cell lysates were prepared for SDS-PAGE, and immunoblots were processed with a custom PBPA or PBP4 polyclonal antibody. The detection of pbp4 in the cell lysates shows increased PBP4 expression in the ΔpbpA strain over that of its parent LS4828 strain. These blots are representative of data from 2 biological replicates. The blots were then stained with Coomassie R-250 for confirmation of equal protein transfer.