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After being treated with HG and Bre (50 or 100 μM) for 48 h, MPC5 cell viability (A) and apoptosis (B and C) were assessed using CCK-8 assays and TUNEL staining, respectively. (D) After being treated with HG and Bre (50 or 100 μM) for 48 h, Bax and Bcl-2 protein levels in MPC5 cells were assessed by western blot analysis. (E) Intracellular ROS levels were assessed in MPC5 cells after treatment with HG and Bre (100 μM). **p < 0.01.
