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. 2023 Feb 13;11:e14826. doi: 10.7717/peerj.14826

Figure 4. Bre decreased NF-κB/NLRP3-mediated pyroptosis in HG-treated podocytes in vitro.

Figure 4

(A and B) After being treated with HG and Bre (50 or 100 μM) for 48 h, the protein levels of p-p65 were assessed in MPC5 cells by western blot analysis. (C) IF analysis was carried out to assess the nuclear translocation of p65 in MPC5 cells after being treated with HG and Bre (50 or 100 μM) for 48 h. (D and E) After being treated with HG and Bre (50 or 100 μM) for 48 h, the protein levels of NLRP3 and activated caspase 1 were assessed in MPC5 cells by western blot analysis. (F) qRT‐PCR analysis of IL-18 and IL-1β mRNA levels in MPC5 cells. (G) Western blot analysis of GSDMD protein levels in MPC5 cells. (H) ELISA analysis of LDH release in MPC5 cells. **p < 0.01.