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Characterization of ND and ND-GSK-J4. (a) Preparation of GSK-J4-loaded nanodiscs (ND-GSK-J4). (b, c) Characterization of ND and ND-GSK-J4 via DLS and TEM (scale bar: 200/50 nm, 100/25 nm). (d) Dio-ND-GSK-J4 were co-incubated with BMSCs or BMDMs. The macrophage target effect in vitro was confirmed using a cellular uptake analysis (scale bar: 10 μm). (e) The bone target effect was confirmed using a Vivo FX PRO imaging system.
