Figure 3.

KDM6B played an integral role in the circadian rhythm of BMDMs. (a) GSK-J4 suppressed the BMAL1 mRNA level in BMDMs, as indicated by RT-qPCR testing. (b) DNA gel electrophoresis results of mouse tail identification. (c, d) Identification of the proportion of macrophages in the bone marrow of wild-type and BMAL-KO mice using flow cytometry and the corresponding statistical analysis. (e, f) Synchronized mature macrophages were stimulated by GSK-J4, and the mRNA and protein expressions of circadian rhythm-related genes were detected by RT-qPCR and WB at specific intervals (circadian time (CT) 0–12: yellow background; CT12–24: gray background).