Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 30;17(3):3153–3167. doi: 10.1021/acsnano.2c12440

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

KDM6B decreased the M1/M2 ratio and promoted BMSC osteogenic differentiation in the BMSCs–BMDMs coculture system. (a) RT-qPCR and WB analyses were performed to analyze the effect of KDM6B and its inhibitor GSK-J4 on the expression of BMAL1 in BMDMs. (b) Flow cytometry was used to detect the effects of KDM6B and its inhibitor GSK-J4 on macrophage polarization. (c) Statistical results of flow cytometry. (d) Immunofluorescence of macrophage polarization and statistical analysis (scale bar: 100 μm). (e) BMDMs or BMSCs were added to the upper or lower chambers of the Transwell plates, and the BMSCs in the lower chamber were osteogenically induced. The osteogenic differentiation of BMSCs in the lower chamber was evaluated via ALP staining or Alizarin Red S staining (scale bar: 100 μm).