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. 2022 Nov 8;2(11):1372–1387. doi: 10.1158/2767-9764.CRC-22-0170

FIGURE 3.

FIGURE 3

Silencing PKCα reduces PC3 cell tumorigenic activity in nude mice. PC3 cells were subjected to stable PKCα depletion using two different shRNA lentiviruses, followed by puromycin selection. Cells were inoculated subcutaneously into nude mice, and tumor formation was followed for the time indicated in the figure. A, Expression of PKC isozymes in the inoculated cell lines, as determined by Western blot analysis. B, Pictures of tumors isolated from all mouse cohorts. C, Time-course analysis of tumor formation Results are expressed as mean ± SD (n = 8). D, Tumor weight was determined after sacrificing mice at day 37 postinoculation with the different PC3 cell lines. Results are expressed as mean ± SD (n = 8). E, Representative IHC images for Ki67, phospho-ERK, and TUNEL. NTC, non-target control shRNA lentivirus. ****, P < 0.0001 versus NTC.