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. 2022 Nov 8;2(11):1372–1387. doi: 10.1158/2767-9764.CRC-22-0170

FIGURE 4.

FIGURE 4

PKCα association with prostate cancer cell line EMT and invasiveness. A, Correlations between PKCα (PRKCA) and EMT markers from the CCLE database. ●, DU145; ▲, PC3; ■, NCI H660; ●, VCaP; ▲, 22RV1; ■, LNCaP. B, Correlations between PKCα (PRKCA) and EMT markers from TCGA-PRAD human dataset (498 patients). D, Representative Western blot analysis for PKCα and EMT markers in prostate cancer cell lines. E, Cell invasion was determined in PC3 and DU145 cells using a Boyden chamber with Matrigel, 48 hours after transfection with siRNA duplexes for PKCα (α1, α2) or NTC. Experiments were done in the absence or presence of 5% FBS in the lower chamber. Left, Representative invasion experiment. Right, Quantification of Boyden chamber migration using 5% FBS. Results are normalized to invasion in parental cells (no siRNA, dotted lines), and expressed as mean ± SEM of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 versus NTC.