PKCα controls the expression of EMT markers in human prostate cancer. A, Classification of primary prostate adenocarcinomas (TCGA-PRAD, n = 498) into “High PKCa” or “Low PKCa” expression levels according to the StepMiner one-step algorithm. B, Heatmap showing the differential gene expression from RNA-seq analysis in comparison with the same genes in “High PKCa” or “Low PKCa” human prostate tumors. C, Heatmaps for the expression of EMT-TFs and mesenchymal markers. Left, Expression upon PKCα RNAi silencing in PC3 cells, as determined by RNA-seq. Right, Expression in “High PKCa” and “Low PKCa” human prostate tumors from TCGA-PRAD. The epithelial marker E-cadherin (CDH1) is also shown. D, Violin plots indicating P values for the comparison of EMT-TF and mesenchymal marker expression in “High PKCa” and “Low PKCa” human prostate tumors from TCGA-PRAD.