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. 2019 Jun 28;11(11):1067–1083. doi: 10.4155/bio-2018-0232

Figure 1. . Representative base peak chromatograms and overlap of peptides and protein groups for 2 μg of HeLa protein digest obtained via nano- and standard-flow ESI.

Figure 1. 

Figure 1. 

(A) Analysis of 2 μg of HeLa protein digest via nano-flow (top) and standard-flow (bottom) ESI revealed well-resolved chromatograms in both approaches, with the nano-flow ESI method having double the analysis time. (A) Proteomic analysis resulted in 6165 ± 14 and 4634 ± 281653 ± 37 peptides and 1178 ± 15 and 826 ± 14 protein groups, respectively. (B) Venn diagram analysis revealed a technical replicate overlap of 3771 peptides and 953 protein groups for 2 μg of HeLa protein digest via nano-flow ESI (top) and a technical replicate overlap of 2577 peptides and 616 protein groups for 2 μg of HeLa protein digest via standard-flow ESI (bottom).