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. 2019 Jun 28;11(11):1067–1083. doi: 10.4155/bio-2018-0232

Figure 3. . Method evaluation and analysis of the differential liver proteome from an 11-week symptomatic NPC1 mouse model.

Figure 3. 

Figure 3. 

(A) Individual liver lysates (N = 3 for each genotype) were subject to electrophoresis and western blotting to evaluate the sensitivity of the standard-flow ESI approach for measuring the differential proteome in the liver tissue of 11-week Npc1+/+ and Npc1-/- mice. Niemann–Pick, type c1, (NpC1), fatty acid binding protein 5 (Fabp5), and fatty acid binding protein 7 (Fabp7) were observed to be increased in Npc1-/- mice relative to Npc1+/+ mice. Intensities are reported as relative to Gapdh. Significance was determined using an unpaired t-test with Welch’s correction. (B) Differential proteins (y-axis) from the liver proteome where enriched for the top 20 matching KEGG pathways (x-axis). (C) Differential proteins (y-axis) from the liver proteome where enriched for the top cellular locations (x-axis).