Figure 5. . Western blot evaluation of lysosome membrane protein 2 and ras-related binding protein 7a at different stages of Npc1.

Individual liver lysates (N = 3 for each genotype) 3-, 5-, 7- and 9-week Npc1^+/+ and Npc1^-/- animals were subject to electrophoresis and Western blotting of Limp2 and Rab7a. Limp2 shows increased expression at all stages of the disease while Rab7a is only significantly altered at 9 and 11 weeks. Intensities are reported as relative to Gapdh. Significance was determined using an unpaired t-test with Welch’s correction.