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. 2023 Jan 12;120(3):e2213317120. doi: 10.1073/pnas.2213317120

Fig. 1.

Fig. 1.

Endothelial-specific Robo4 overexpression suppresses LPS-induced mortality and melanoma cell migration in mice. (A) Transgenes and genotyping results of Robo4iEC mice. CDH5 promoter induces endothelial-specific expression of Cre/ERT2. Tamoxifen activates Cre/ERT2 and induces deletion of the floxed Stop codon and CAG promotor-driven mRobo4 overexpression. (B) Expression level of Robo4 mRNA in the organs of Robo4iEC mice treated with tamoxifen (n = 5, *P < 0.05, **P < 0.01 by the unpaired t test) (C) Vascular permeability in the lungs in the Robo4iEC and control (CDH5-Cre/ERT2) mice. (n = 6, *P < 0.05 by the unpaired t test) (D) Experimental metastasis models using Robo4iEC and control mice treated with tamoxifen. B16-F10 melanoma cells were intravenously injected, and metastasized colonies were counted (n = 6, *P < 0.05 by the unpaired t test). (E) Survival study using LPS-injection models in Robo4iEC and control mice treated with tamoxifen (control: n = 18, Robo4iEC: n = 17; P = 0.017 by the log-rank test) Data are expressed as the mean ± SEM.