Fig 3. Effect of deleting atxA on capsule production after a short (5h) incubation under different growth conditions.

Spores of the wild type and ΔatxA mutant (top panel) were seeded into 100μl of sDMEM as is or supplemented with 10% NRS and incubated at 37°C in an ambient or 10% CO₂ atmosphere (as indicated on the right) for 24h. Capsule was imaged by India ink negative staining (capsule presence forms a typical bright outer layer).