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. 2023 Feb 16;18(2):e0281879. doi: 10.1371/journal.pone.0281879

Fig 5. Effect of AtxA on capsule production in the presence of either AcpA or AcpB.

Fig 5

Spores of the different mutants (top panel) were seeded into 100μl of sDMEM as is or supplemented with 0.75% HCO3- or 10% NRS and incubated at 37°C in 10% CO2 atmosphere (as indicated on the right) for 24h. Capsule was imaged by India ink negative staining (capsule presence forms a typical bright outer layer).