Table 4. Gas analysis of the sDMEM media under the different growth conditions.
| 10% CO2 | - | + | + | - | + | Normal arterial | Normal venous |
|---|---|---|---|---|---|---|---|
| Supplement | - | - | 10% FCS | 10% FCS | 0.75% HCO3 1:1* | ||
| pH | 8.0 | 7.691 | 7.447 | 8.149 | 7.972 | 7.35–7.45 | 7.31–7.41 |
| PCO2 (mmHg) | 18.4 | 34.9 | 58.1 | 14.2 | 34.3 | 35–45 | 41–51 |
| PO2 (mmHg) | 230 | 148 | 126 | 173 | 183 | 80–100 | 35–40 |
| HCO3 (mmol/L) | 48.7 | 42.2 | 40.1 | 49.5 | 79.2 | 22–26 | 22–26 |
| sO2 (%) | 100 | 100 | 99 | 100 | 100 | 95–100 | / |
*at the end of the 4h incubation the media was diluted 1:1 in sDMEM prior to the analysis since the undiluted media was off scale.