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. 2023 Feb 16;18(2):e0281464. doi: 10.1371/journal.pone.0281464

Fig 3. Projections from dlPAG to the cuneiform nucleus.

Fig 3

(A) Vglut2+ cells in dPAG project to the cuneiform nucleus (CnF) and to the superior lateral parabrachial nucleus (LPBS). Cre-dependent Synaptophysin-iRFP expressing virus was injected in dPAG of a Vglut2::Cre transgenic animal. Sections show synaptic boutons expressing Synaptophysin-iRFP in CnF and LPBS (IC, Inferior Colliculus; Blue, DAPI). (B) Graphical representation of experimental strategy for retrograde labeling with CTB and identification of glutamatergic (Vglut2+) or GABAergic (Gad2+) identity of projection neurons. (C) Cell bodies of CnF afferents in PAG were limited to the dorsolateral column (green, CTB; left, CnF; right, PAG; blue, DAPI; scale bar, 250 μm). (D) CTB label (green) in PAG co-localized with both (left) glutamatergic (red) and (right) GABAergic (red) neurons (scale bar, 50 μm).