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. 2023 Feb 6;19(2):e1011135. doi: 10.1371/journal.ppat.1011135

Fig 3. The impact of L204M mutation on NA activity of different subtypes of influenza viruses.

Fig 3

Shown is the specific impact of L204M mutation on NA activity of different subtypes of influenza viruses carrying group 1 and group 2 NA against small substrate MUNANA (A-I) and large substrate fetuin (J). PR8-NA5, PR8-NA6 and PR8-NA8 were generated using seven gene segments from PR8 and NA from clade 2.3.4.4 H5N5, H5N6 or H5N8, respectively (see S1 Table). Viruses were adjusted to 32 HA units using chicken erythrocytes. The p-values shown in the graphs refer to the statistical significance of the 50% NA-activity (shown as dashed lines) of different 204-mutants compared to the wild-type viruses (A-I). The activity of different L204M-carrying viruses against fetuin in ELLA assay was calculated relative to the same virus dilution of the wild type viruses. Asterisks refer to statistical significance at p value < 0.05 as indicated (A-I) or compared to the NA activity of the wild type viruses in each subtype (J). ELLA-Assay was done in three independent runs and the results are expressed as mean and standard deviation of all replicates (black bars).