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[Preprint]. 2023 Feb 8:2023.02.07.527364. [Version 1] doi: 10.1101/2023.02.07.527364

Figure 4. Fibroblast-derived cytokine and chemokine promote iCM survival after erastin treatment.

Figure 4.

(A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of iCMs, cultured in control medium (DMEM), HCF conditioned medium, or H2O2-treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H2O2-treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E, F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. Error bars indicate SD. *, p<0.05; **, p<0.01. NS, not significant.