Figure 2.

shFXN have upregulated oxidative stress. (A) EVEC and (B) shFXN are incubated with 0.5µM CellRox Deep Red and 0.7µg/ml Hoechst for 30 minutes at 37°C. (C) Fluorescence values are read on the Cytation-5 (Biotek), the Cellrox normalized to Hoechst values, and then normalized to EVEC values to represent a fold change. Student’s T-test, α=0.05. *; p<0.05. EVEC and shFXN n=8. Representative of 3 biological replicates. The images in panels A and B were cropped from their original size while maintaining the scale of the scale bar.