Figure 6.

Paracellular permeability is increased in shFXN. (A) hBMVEC plated in the apical chamber of transwells are treated with 50µM Lucifer Yellow (LY). Transendothelial electrical resistance (TEER) measurements and LY aliquots are taken at 24, 48, 72, and 96-hours in culture. (B) TEER is measured using the Endohm-6G (World Precision Instruments), sample wells are background corrected to a transwell not containing cells. Blank-corrected values are multiplied by the growth area of the 24-well transwell (3.36cm2) and normalized to the final protein content of the well. Represented as fold change to EVEC per timepoint. (C) Media from the basal chamber is analyzed for nanomoles of LY fluxed at 24–96 hours in culture at 490nm and 525nm excitation and emission, respectively. The cell monolayers were lysed after the final timepoint, and total protein content was used for normalization of all experiments. Student’s T-test α =0.05; * p < 0.05, ** p < 0.01, *** p < 0.001. (B) EVEC; n =11, and shFXN; n = 12. (C) EVEC; n = 8, and shFXN; n = 7. Representative of 2–4 biological replicates.