Fig. 3. Mutations in the orthologs of ITFG1 interactors resulted in phenotypes similar to that of lnkn-1 mutants.

(A) Homozygotic animals of lethal/ sterile mutations used in this study descended from balanced heterozygotic mothers. Homozygotic animals were identified through the lack of fluorescently labeled balancers (green in the cartoon). In the maternal effect experiments, the homozygotic hermaphrodites were mated with wild-type males carrying a fluorescent genetic marker (oxTi719 [eft-3p::tdTomato::H2B]) (Frokjaer-Jensen et al., 2014). (B) LNKN-1 is required maternally. Heterozygotic cross-progenies of lnkn-1(sy1596) hermaphrodites and lnkn-1(+/+) males fail to develop and die without hatching (The embryos carrying the orange-colored male-derived oxTi719 marker can be seen). (C) Phenotypical analysis of the orthologs of ITFG1 interactors in C. elegans. Phenotype 2 and 5 was defined by having F₁ progenies that hatch; Phenotype 3 was defined by embryos/ embryo-like objects or oocytes observed outside of the unmated hermaphrodite. Embryos and, in particular, oocytes may not be completely absent in all animals. This was especially the case in unc-94(ok1210). Some rod-1(tm6186) animals also produce oocytes and embryo-like objects. Phenotype 4 was defined by the observation of F₁ embryos/animals carrying the male-derived oxTi719 marker. *: Very rarely; #: maternal effect sterile.